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KMID : 0381120200420010025
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Genes and Genomics
2020 Volume.42 No. 1 p.25 ~ p.39
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Cytological, genetic, and proteomic analysis of a sesame (Sesamum indicum L.) mutant Siyl-1 with yellow?green leaf color
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Gao Tong-Mei
Wei Shuang-Ling
Chen Jing
Wu Yin
Li Feng
Wei Li-Bin
Li Chun
Zeng Yan-Juan
Tian Yuan
Wang Dong-Yong
Zhang Hai-Yang
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Abstract
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Background: Both photosynthetic pigments and chloroplasts in plant leaf cells play an important role in deciding on the photosynthetic capacity and efficiency in plants. Systematical investigating the regulatory mechanism of chloroplast development and chlorophyll (Chl) content variation is necessary for clarifying the photosynthesis mechanism for crops.
Objective: This study aims to explore the critical regulatory mechanism of leaf color mutation in a yellow?green leaf sesame mutant Siyl-1.
Methods: We performed the genetic analysis of the yellow-green leaf color mutation using the F2 population of the mutant Siyl-1. We compared the morphological structure of the chloroplasts, chlorophyll content of the three genotypes of the mutant F2 progeny. We performed the two-dimensional gel electrophoresis (2-DE) and compared the protein expression variation between the mutant progeny and the wild type.
Results: Genetic analysis indicated that there were 3 phenotypes of the F2 population of the mutant Siyl-1, i.e., YY type with light-yellow leaf color (lethal); Yy type with yellow-green leaf color, and yy type with normal green leaf color. The yellow-green mutation was controlled by an incompletely dominant nuclear gene, Siyl-1. Compared with the wild genotype, the chloroplast number and the morphological structure in YY and Yy mutant lines varied evidently. The chlorophyll content also significantly decreased (P?
Conclusion: The results revealed the genetic character of yellow-green leaf color mutant Siyl-1. 98 DEPs were found in YY and Yy mutant compared with the wild genotype. The regulation pathway related with the yellow leaf trait mutation in sesame was analyzed for the first time. The findings supplied the basic theoretical and gene basis for leaf color and chloroplast development mechanism in sesame.
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KEYWORD
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Sesame, 2-DE, Proteomic, Yellow?green leaf mutant, Photosynthesis
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